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KMID : 0854720120320010043
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Korean Journal of Asthma, Allergy and Clinical Immunology
2012 Volume.32 No. 1 p.43 ~ p.50
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Anti-Inflammatory Effect of Houttuynia Cordata Thunb in Activated Raw 264.7 Cells Activated with Lipopolysaccharide and Interferon Gamma
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Cho Wan-Ik
Kim Hyun-Kyu
Park Ju-Hee
Park Kui-Young
Lim Yun-Young
Kim Young-Heui
Kim Ki-Ho
Kim Hyeong-Mi
Li Kap-Sok
Kim Beom-Joon
Seo Seong-Jun
Kim Myeung-Nam
Hong Chang-Kwun
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Abstract
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Background:Houttuynia cordata has long been used in traditional oriental medicine for the treatment of inflammatory disease. In the present study, we investigated the anti-inflammatory effect of Houttuynia cordata extracts on Raw 264.7 cells, which is activated with lipopolysaccharide (LPS) and interferon-γ (IFN-γ).
Methods:We treated LPS-and IFN-γ-activated murine macrophage Raw 264.7 cells with extracts of Houttuynia
cordata and measured the production of nitric oxide (NO) and prostaglandin E2 (PGE2), and the expression of inducible
nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, and pro-inflammatory cytokines (Interleukin [IL]-1α, IL-1β,
IL-4, IL-6, IL-10, IL-12, tumor necrosis factor-α, regulated on activation normal T-cell expressed and secreted) in activated
Raw 264.7 cells after treatment with Houttuynia cordata extracts.
Results:NO formation by activated Raw 264.7 cells was reduced in response to Houttuynia Cordata in a dose-dependent manner. Formation of PGE2, a final product of the COX pathway was reduced by Houttuynia cordata extracts and reduction in COX-2 expression was proportional to the reduction in PGE2. The expression of proteins, iNOS and COX-2 decreased with increasing concentration of Houttuynia cordata extracts. The related pro-inflammatory cytokines did not decrease in the cells treated with Houttuynia cordata extracts.
Conslusion:Houttuynia Cordata extracts suppressed the production of NO and PGE2 and decreased the expression of iNOS and COX-2. It has potential for the treatment of inflammatory diseases. Further studies are needed to elucidate the effects of Houttuynia Cordata extracts on the production of inflammatory cytokines.
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KEYWORD
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Cyclooxygenase-2, Houttuynia cordata, Inducible nitric oxide synthase
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